Journal: Journal of Cancer

Article Title: FAM188B promotes progression of hepatocellular carcinoma by regulating YAP/TAZ via interaction with USP10

doi: 10.7150/jca.125659

Figure Lengend Snippet: FAM188B expression is upregulated and associated with poor prognosis in hepatocellular carcinoma (HCC). (A) Expression of FAM188B mRNA in tumor and non-tumor tissues and its association with cancer stage and tumor grade in UALCAN database. (B) The expression of FAM188B protein was evaluated in normal hepatocytes (LX-2) and in hepatocellular carcinoma (HCC) cells (MHCC97H, Huh7, Hep3B and SNU387) by western blot. (C) Validation of FAM188B expression after overexpression in the indicated cell lines using western blotting (n = 3). (D) Proliferation of MHCC97H and Huh7 cells, as detected by performing CCK-8 assays (n = 10). (E) Migratory and invasive capacities of MHCC97H and Huh7 cells within 24 hours, as evaluated using Transwell assays (scale bar, 50 μm) (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns, not significant. The data are expressed as the mean ± SD of three independent experiments.

Article Snippet: Human liver tumor-derived cell lines SNU387 and Hep3B, human embryonic kidney HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) in 2021.

Techniques: Expressing, Western Blot, Biomarker Discovery, Over Expression, CCK-8 Assay

Journal: Journal of Cancer

Article Title: FAM188B promotes progression of hepatocellular carcinoma by regulating YAP/TAZ via interaction with USP10

doi: 10.7150/jca.125659

Figure Lengend Snippet: FAM188B interacts with USP10. (A) Schematic illustration of IP and GO enrichment of identified proteins (using all proteins in the species database as the background). Fisher's exact test was used to analyze the significance and P value < 0.05 were considered significant. (B) Expression levels of USP10 mRNA in tumor (n = 374) and non-tumor (n = 50) tissues and its relationship with FAM188B, based on a public database. (C) The protein expression level of USP10 was evaluated in normal hepatocytes (LX-2) and hepatocellular carcinoma (HCC) cells (MHCC97H, Huh7, Hep3B and SNU387) by western blot. (D) Localization of USP10 in HCC cells (scale bar, 20 μm). (E) Co-IP assays to confirm the protein-protein interaction between FAM188B and USP10 in vitro . **p < 0.01, ***p < 0.001. The data are expressed as the mean ± SD of three independent experiments. IP, immunoprecipitation. Co-IP, co-immunoprecipitation.

Article Snippet: Human liver tumor-derived cell lines SNU387 and Hep3B, human embryonic kidney HEK293T cells were obtained from the American Type Culture Collection (Manassas, VA, USA) in 2021.

Techniques: Expressing, Western Blot, Co-Immunoprecipitation Assay, In Vitro, Immunoprecipitation

Journal: Naunyn-Schmiedeberg's Archives of Pharmacology

Article Title: Morin hydrate alleviated cisplatin-induced testicular toxicity in rats via modulating NLRP3/NF-κB pathway

doi: 10.1007/s00210-025-04867-5

Figure Lengend Snippet: The MTT cell viability assay showing the cytotoxicity of Cis, MH, and their combination in A the human prostate (PC3), B breast (MCF7), and C liver (HepG2) cancer cell lines. In this dose–response curve, the average percentage viability was plotted on the y -axis against the logarithm of the concentration for each treatment on the x -axis, and the IC 50 (the concentration corresponding to the 50% viability) was calculated

Article Snippet: Human prostate (PC3), breast (MCF7), and liver (HepG2) cancer cell lines were obtained from American Type Culture Collection (ATCC) (VA, USA; Catalog No. CRL-1435, Catalog No. HTB-22, and Catalog No. HB-8065, RRID: CVCL_0027), respectively.

Techniques: Viability Assay, Concentration Assay